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. 2017 Feb 17;31(6):962–971. doi: 10.1038/eye.2017.8

Figure 1.

Figure 1

Ex vivo cultivation of human CESCs in a HA hydrogel scaffold-based culture system. Fresh limbal explant was trimmed and dissected into ~2 mm × 2 mm sized pieces and placed onto the HyStem-C Hydrogel-coated culture inserts in 12-well plates. Two culture media were used. Group A used traditional SHEM including toxin A and 5% FBS. Group B used defined SHEM without toxin A and 5% FBS, but included 20% serum replacement (Knockout SR, Thermo Fisher Scientific). The HyStem-C Hydrogel has a good stiffness and can be manipulated with forceps.