Figure 3.

The histology of the tissue generated on hydrogel scaffolds by cross-section with HE staining. At the early stage of cultivation around 1 week, a single cell layer cover the scaffold surface was observed in both media. At the end of cultivation, the cultured cells formed an artificial epithelium of 5–7 cellular layers in group A, whereas less cellular layers (3–5 layers) were observed in group B. The cellular layer(s) cultured in group B seemed less organized and striated than those cultured in group A.