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. 2017 May 4;116(12):1595–1603. doi: 10.1038/bjc.2017.131

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Copyright © 2017 Cancer Research UK

From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Presence of functional RSPO fusion transcripts in BC cells. (A) Detection of EIF3E (exon1)-RSPO2 (exon2) and EIF3E (exon1)-RSPO2 (exon3) fusion transcripts (351 pb and 88 pb, respectively, see Supplementary Table S3) in HBCx-15 TNBC PDX and its derived HBCc-15 cell line and BT549 cell line. (B) Detection of RSPO2 protein in HBCx-15 TNBC PDX and HBCx-3 (negative control, TNBC PDX, which does not express RSPO2). HBCx-3 TNBC PDX (left panel) and HBCx-15 TNBC PDX (right panel) blocks were prepared and immunostained with anti-RSPO2 antibody. Magnification × 20 (top panels) and × 40 (bottom panels).