Figure 4.

Effect of inhibition of RSPO2. (A) Inhibition of proliferation of BT549 cells by two siRNA of RSPO2. (B) Inhibition of mRNA expression determined by qRT–PCR: RSPO2 expression was decreased by siRSPO2-1 and siRSPO2-2 to 5% and 35%, respectively, vs CTL (control). *P<0,0001, Mann–Whitney test. (C) The Wnt/β-catenin inhibitor IWR-1 inhibits the growth of HBCx-60 RSPO2-overexpressing human TNBC PDX; ▪=IWR-1, ○=vehicle alone. Tumour growth was evaluated by plotting the mean RTV±s.d. per group over time after first treatment. *P<0.05, unpaired t-test.