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. 2017 Jan 25;42(8):1657–1669. doi: 10.1038/npp.2017.6

Figure 1.

Figure 1

Regulation of Tet1 expression by CSDS. (a) Tet1 mRNA levels are decreased in the NAc of susceptible mice 48 h after CSDS. (Con: control, Sus: susceptible, Res: resilient. N=8 for each group. Two-tailed t-test, *P=0.025.) (b) Schematic of floxed Tet1 locus (Dawlaty et al, 2011). Open boxes indicate exons of Tet1 gene, black triangles represent loxP sites flanking exon 4, which is excised in the presence of Cre. (c) qPCR validation of Tet1 decrease after AAV-Cre injection in NAc of floxed Tet1 mice (Cre) as compared with AAV-GFP controls (Con). Two primer sets were used, which cover exon 4 alone (Tet1 primer set 1) or both exons 4 and 5 (Tet1 primer set 2) (N=7 for each group. Two-tailed t-test, *P=0.011, **P=0.005). (d) RNAseq read counts of Tet1. Schematic of relative position of exons 4 and 5 is shown on the bottom. Blue traces represent normalized read counts across these three exons in both control (Con) and Cre conditions under the same scale. Red box highlights differential reading of exon 4.