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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Cancer Ther. 2017 May 3;16(7):1324–1334. doi: 10.1158/1535-7163.MCT-16-0685

Figure 5. Differential signaling effects of Gp2 variants on insulin/IGF signaling in breast cancer cells.

Figure 5

Cells were plated, serum-starved overnight, pre-treated with indicated concentrations of Gp2 overnight for (A) MCF-7L, (B) MCF-7L TamR and 4 hours Gp2 pretreatment for (C) T47D, (D) T47D TamR before treating with either 10 nM IGF-I, 10 nM IGF-II or 10 nM insulin for 15 minutes. Whole cell lysates were separated by SDS-PAGE and immunoblotted with indicated antibodies.