Fig 1. Severity of infection induced by mastitis E. coli strains in mice after intra-mammary inoculation.
Mice were inoculated with 103 cfu per gland in the right 4th mammary gland and with an equal volume of PBS in the left 4th mammary gland (control gland, CTL). A, percentages of mice by clinical scores. B, cfu were counted 24 and 48 h post-inoculation in the infected mammary gland. C, neutrophil recruitment in the infected glands and control glands was measured by FACS of mammary cells labelled with anti-CD45 and anti-Ly6G. D and E, mediators of inflammation were quantified by ELISA (D, MIP-2a; E, IL-6). Data presented are values from each individual animal with medians and interquartile ranges indicated as vertical bars. * indicates statistical significance (P < 0.05). P-values were calculated using exact permutation tests after global comparison using a Kruskal and Wallis test. A: comparison against MG1655; B, C, D: comparison against 4th right mock-infected gland.