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. 2017 Jun 9;49(6):e344. doi: 10.1038/emm.2017.74

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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The SSEA1⁺ population from U373MG glioma cells was more clonogenic than the SSEA1⁻ population. (a) The purity of the SSEA1⁺ population sorted by immunomagnetic selection was confirmed by flow cytometer. (b) Representative cell morphology of each population three days after cell sorting is shown. (c) Total numbers of colonies were determined in SSEA1⁺ versus SSEA1⁻ populations using the soft-agar colony-forming assay. (d) Numbers of wells without tumor spheres were quantified in SSEA1⁺ versus SSEA1⁻ populations using the limiting dilution sphere-forming assay (primary). Serial secondary sphere forming assay was performed (secondary). **P<0.01 (Student’s t-test). Scale bar=50 μm.