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. 2017 Jun 9;49(6):e344. doi: 10.1038/emm.2017.74

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Nicotinamide adenine dinucleotide (NAD⁺) levels, SIRT3 expression and activity, and lactate production in SSEA1⁺ and SSEA1⁻ populations from U373MG glioma cells are shown. (a) SSEA1⁺ and SSEA1⁻ populations were sorted from U373MG glioma cells (total, unfractionated) and then fractionated into mitochondrial, nuclear and cytosolic fractions. The NAD⁺ levels in each lysate were quantified. (b) Western blot analysis of SIRT1, 2 and 3 in total protein of each subpopulations. β-Actin was used as an internal control. (c) SIRT3 enzyme activity and (d) lactate contents in each population were determined. **P<0.01; ***P<0.001 (Student’s t-test).