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. 2017 Jun 9;49(6):e344. doi: 10.1038/emm.2017.74

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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The tumorigenic potential of nicotinamide nucleotide transhydrogenase (NNT) overexpression or knockdown in SSEA1⁺ tumor-initiating cells is shown. (a) Control (Con), retroviral NNT-transduced or short interfering RNA for NNT (siNNT)-transfected U373MG SSEA1⁺ cells were injected subcutaneously and tumors were excised at 6 weeks after injection. Representative images of excised tumor mass in each group were shown. (b) Tumor volume was measured daily after tumor mass detected. (c) Intracranial orthotopic injection of control or siNNT-transfected U373MG SSEA1⁺ cells. Representative hematoxylin and eosin images of each two mice brain (upper and lower) and higher magnification of each section (each right in group). (d) Tumor area was quantified. (e) Immunohistochemistry of Sox2 and Nestin in each brain. 4,6-Diamidino-2-phenylindole (DAPI) staining (blue) was used to identify nuclei. *P<0.05; **P<0.01; ***P<0.001 (Student’s t-test).