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. 2017 Jun 23;49(6):e348. doi: 10.1038/emm.2017.80

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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The effect of SMG on hepatocyte proliferation. (a) Immunostaining analysis of Ki67 (left panels), PCNA (center panels) and PH3 (right panels) in the rat liver of the control, TS-14, TS-28 and TS-42 groups. Scale bar, 50 μm. (b) Quantification of the Ki67-, PCNA- and PH3-positive cell number in the liver of the control, TS-14, TS-28 and TS-42 groups, n=6. (c) RT-qPCR analysis of gene expression related to cell cycle regulation such as CCNA2, CCND1, CDK1, CDK2 and FOXM1 in the rat liver of the control and TS groups. n=6. (d) Protein levels of PCNA, cyclin D1, cyclin D3 and CDK2 were analyzed by western blotting. n=3. The intensity of the protein bands was semi-quantitatively analyzed using ImageJ software. *P<0.05, ^**P<0.01 compared to Control. SMG, simulated microgravity; TS, tail suspension.