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. 2017 Jun 23;49(6):e348. doi: 10.1038/emm.2017.80

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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miR-223 suppressed Hepa1-6 cell proliferation in vitro. (a) The relative viability of Hepa1-6 cells was assessed with the MTS assay at different time points after transfection with miR-223 mimic or negative control. (b) Protein expression of the proliferation regulators PCNA and cyclin D1 in Hepa1-6 cells was examined 48 h after transfection with miR-233 mimic or the negative control by western blotting and semi-quantitatively analyzed using ImageJ software. *P<0.05, ^**P<0.01 compared to Control.