Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 23;49(6):e348. doi: 10.1038/emm.2017.80

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

PMC Copyright notice

CDK2 and CUL1 were the direct targets of miR-223. Schematic representation of the luciferase constructs used for reporter assays. The miR-223 target sites within the 3′-UTR of CDK2 (a) and CUL1 (b) are shown as black boxes. The sequences below indicated the putative miR-223 target sites on the wild-type (WT) and mutated (MUT) constructs. The miR-223-binding sites and seed sequences are marked with bold italics. (c, d) Effects of miR-223 on the luciferase activity of the reporter gene in the WT or mutated vector in Hepa1-6 cells. n=3. (e) CDK2 and CUL1 protein expression in Hepa1-6 cells was examined after transfection with miR-233 mimic and negative control for 48 h and semi-quantitative evaluation using ImageJ software. mRNA (f) and protein (g) expression of CUL1 analyzed in the rat liver of the control and TS groups. n=6, *P<0.05, ^**P<0.01 compared to Control.