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. 2017 Jun 23;49(6):e348. doi: 10.1038/emm.2017.80

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Upregulation of p27 expression in vitro and in vivo. (a) Immunofluorescence staining of p27 in the rat liver of the control, TS-14, TS-28 and TS-42 groups. Images were captured by a Leica TCS SP5 confocal scanning laser microscope. p27 in red (Cy3); nuclei in blue (DAPI). Scale bar, 50 μm. (b) Quantification of the number of p27-positive cells in the liver of the control, TS-14, TS-28 and TS-42 groups. n=6. (c) p27 protein expression level in the TS liver was significantly elevated compared with that of the control as shown by western blotting and semi-quantitative evaluation using ImageJ software. (d) The expression of the p27 protein in Hepa1-6 cells was examined 48 h after transfection with miR-233 mimic and negative control by western blotting and semi-quantitatively evaluated using ImageJ software. *P<0.05, ^**P<0.01 compared to Control. (e) Diagram illustrating the regulatory hierarchy among miR-223 and its targets CDK2 and CUL1 in the control of hepatocyte proliferation.