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. 2017 Jul 20;12(7):e0180844. doi: 10.1371/journal.pone.0180844

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© 2017 Liu et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Overexpression or silencing of HMGB1 expression in A459 NSCLC cells by cloning (LV-HMGB1) or siRNA transfection, respectively, was examined by real-time polymerase chain reaction (PCR) (A and B), western blotting (C and D), and immunocytochemical staining (E). *p <0.05 as compared to the control group. α-Tubulin was used as a loading control. Scale bar = 50 μm.