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. 2017 Jul 20;12(7):e0181597. doi: 10.1371/journal.pone.0181597

Fig 5. Loss of Ramp2 alters mRNA expression of RAMP2-associating GPCRs, including Pthr1.

Fig 5

(A) mRNA expression of RAMP2-interacting GPCRs from e13.5 wild type and Ramp2-/- placentas (n≥3 placentas per genotype). (B) PTH1R protein expression by immunoblot of e13.5 tissue lysates from wild type and Ramp2-/- placentas. (C and D), Immunofluorescence for PTH1R in the labyrinth of wild type and Ramp2-/- placentas. Scale bars, 100 μm. Pth1r, parathyroid hormone receptor 1; Casr, calcium-sensing receptor; Ctr, calcitonin receptor; Calcrl, calcitonin receptor-like receptor; Gcgr, glucagon receptor; Vpac1r, vasoactive intestinal peptide receptor 1; Crhr1, corticotrophin releasing hormone receptor 1. *p<0.01.