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. 2017 Jun 12;6(6):e346. doi: 10.1038/oncsis.2017.42

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Copyright © 2017 The Author(s)

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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PRH levels influence mammosphere formation. MCF-7 control and PRH KD cell lines were induced with IPTG for 7 days then analysed by flow cytometry. (a) Flow cytometry analysis of the CSC markers CD24 and CD44. (b) Quantification of CD24^loCD44^hi cells (n=4, Student’s t-test, ***P<0.005). (c) Quantification of primary mammospheres formed by control and PRH KD MCF-7 cells (n=3, Student’s t-test, *P<0.05). (d) Limiting dilution analysis with secondary MCF-7 control and PRH KD mammospheres counted at day 14, (n=5). (e) Mammospheres and mammosphere-like particles were imaged under optical light (mag. × 10). (f) Quantification of primary mammospheres formed by control and PRH overexpressing MCF-7 cells (n=3, Student’s t-test, *P<0.05).