Figure 5.

PRH overexpression decreases tumour growth. (a) Left—number of 4T1L cells 6 days after infection with Ad-PRH. Cells infected with Ad-PRH or empty adenovirus (Ad) were plated at equal numbers at 24 h and viable cells were counted at day 6 post-infection (n=3, Student’s t-test *P<0.05). Right—Western blot with 4T1L cells infected with Ad or Ad-PRH for 48 h. Lamin A/C as a loading control. (b) Tumour volumes as calculated by caliper measurements (width × height × depth) for each time point following injection of Ad-PRH or Ad-infected 4T1L cells into BALB/C mice (n=10, Student’s t-test, P<0.001 at day 22, combined data from two independent experiments). (c) Tumours were excised from the mice in (b) and weighed (n=10, **P<0.01). (d) Kaplan–Meier survival plot for PRH expression and probability of survival: black=low expression, grey=high expression. Logrank test P=1.1−10⁻¹⁵.