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. 2017 Jun 12;6(6):e348. doi: 10.1038/oncsis.2017.45

Figure 6.

Figure 6

The levels of USP17, USP4 and HAS2 are higher in malignant compared to normal cells, and the knockdown of USP17 or USP4 differentially affects hyaluronan production. (a) Normal human lung fibroblasts (NHLF), breast epithelial cells (MCF10A), as well as breast cancer (MCF7, HS578T, MDA-MB-231-BM) and lung cancer (H1299 and A549) cells were subjected to immunoblotting by specific antibodies against USP17, USP4 and HAS2; the immunodetection of USP17 was preceeded by immunoprecipitation. Data represent one out of three experiments with similar results. (b) USP17 or USP4 were silenced in MDA-MB-231-BM, and the secreted hyaluronan in the culture media was measured by a hyaluronan assay. The inserts depict immunoblots with anti-USP17 or anti-USP4 antibodies to confirm their knockdown efficiencies. The graphs represent the average of three experiments±s.e.m. *P<0.05 and **P<0.01, calculated with unpaired Student’s t-test.