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. 2017 Mar 27;38(7):998–1008. doi: 10.1038/aps.2016.175

Figure 3.

Figure 3

AS-IV attenuates FFA-induced lipid accumulation in hepatocytes. HepG2 cells and primary murine hepatocytes exposed to FFAs (1 mmol/L) were treated with or without AS-IV (100 μg/mL) for 24 h. (A) Representative images of Oil red O staining at 400× magnification in HepG2 cells. (B) Quantification of intracellular lipid content by Oil red O staining in HepG2 cells. (C) Representative images of Oil red O staining at 400× magnification in primary murine hepatocytes. (D) Quantification of intracellular lipid content by Oil red O staining in primary murine hepatocytes. Data are presented as the mean±SEM of three independent experiments (n=3). *P<0.05, **P<0.01 compared with the control group; ##P<0.01 compared with the FFA group. FFAs, free fatty acids; AS-IV, astragaloside IV.