Figure 1.

DATS suppressed cell viability, induced cell cycle G₂/M phase arrest and regulated cell cycle-related protein expression in BGC-823 cells. (A) Effects of DATS on viability in BGC-823 and GSE-1 cells. (B and C) PI staining and flow cytometric analysis were performed on BGC-823 cells after 24 h incubation at the indicated concentrations. Percentages of cells in the G₁, S and G₂/M phases, and representative images are indicated. (D) Representative Western blots from three individual experiments. Quantification analysis is shown for the effect of DATS treatment on cyclin expression in BGC-823 cells. The data are presented as the mean±SEM (n≥3). ^*P<0.05, ^**P<0.01.