Figure 2.

DATS induced BGC-823 cell apoptosis by regulating apoptosis-related protein expression. (A) Annexin V-FITC and PI staining and flow cytometry analysis for apoptosis were performed on BGC-823 cells after 24 h incubation with DATS at the indicated concentrations. Representative images from four independent experiments are indicated. The percentage of early and late apoptotic cells was analyzed by CXP software. (B) Western blot analysis of Bcl-2 family, p53 and cytochrome c protein expression after DATS treatment. The data are presented as the mean±SEM (n≥3). ^*P<0.05, ^**P<0.01.