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. 2017 May 15;38(7):1024–1037. doi: 10.1038/aps.2017.15

Figure 1.

Figure 1

Structure of W2476 and its characterization in BG73, CL108 and INS-1E cells. (A) Chemical structure of W2476 (C15H15N5O2, MW: 297.31). BG73 or CL108 used for counter screening were treated with either 19.4 mmol/L or 3.3 mmol/L glucose with or without different concentrations of W2476 for 24 h. The reporter gene activity was evaluated and the modulating activity of W2476 was examined under high glucose (HG, 19.4 mmol/L) and basal glucose (BG, 3.3 mmol/L) conditions in both BG73 (B) and CL108 (C) cells. INS-1E cells were treated with 10 μmol/L W2476 in the presence of 5 mmol/L or 25 mmol/L glucose for 6, 12, 24 or 48 h (D), or with different doses of W2476 for 24 h (E, F), followed by immunoblotting of TXNIP. Values represent means±SEM for three independent experiments. **P<0.01 vs 5 mmol/L glucose. ##P<0.01 vs25 mmol/L glucose.