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. 2017 May 15;38(7):1024–1037. doi: 10.1038/aps.2017.15

Figure 2.

Figure 2

Effect of W2476 on high glucose-induced INS-1E cell apoptosis. INS-1E cells were incubated at 5 mmol/L or 33.3 mmol/L glucose in the presence or absence of varying concentrations of W2476 with 2% FBS for 48 h. (A, B) Protein expression levels of TXNIP were measured by Western blotting. (C, D) Cell apoptosis was assessed by flow cytometry using Annexin V/PI staining. Immunoblotting of cleaved caspase-3 (E, F) and caspase-3 (E, G) as well as cleaved caspase-9 (H, I) and caspase-9 (H, J) after treatment with W2476. Representative immunoblots were shown and protein contents were separately quantified. All values are presented as mean±SEM of at least three independent experiments; *P<0.05, **P<0.01 vs 5 mmol/L glucose; #P<0.05, ##P<0.01 vs33.3 mmol/L glucose.