Figure 4.
Protective effect of W2476 on STZ-induced diabetic mice. Male C57BL/6 mice received either verapamil in drinking water (100 mg·kg−1·d−1) or an oral dose of W2476 (200 mg·kg−1·d−1) between d 1 and 17. Three days after the treatment, all groups received daily intraperitoneal injection of STZ (40 mg·kg−1·d−1) for 5 d. (A) Blood glucose levels at indicated times minus baseline values measured on d 3 (n=12 per group). (B, C) Effects of W2476 and verapamil on pancreatic islet TXNIP protein levels in each group. On d 17, OGTT was performed to assess blood glucose levels (D) and the glucose area-under-the-curve integrated from 0 to 120 min (AUC0–120; E) in all treatment groups. (F) Immunofluorescence staining of pancreatic islets prepared from three groups after 17-d treatment. Red, glucagon; green, insulin; blue, nuclei/DAPI. Scale bar, 30 μm. Original magnification, 10× (upper panel) and 60× (lower panel). Data shown are mean±SEM. *P<0.05, **P<0.01 vs STZ group.