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. 2017 Apr 17;38(7):1038–1047. doi: 10.1038/aps.2017.8

Figure 2.

Figure 2

Loss of 53BP1 alleviates cell cycle arrest and apoptosis. MDA-MB-436 cells (53BP1+/+) and the #26 clonal variant (53BP1−/−) were treated with simmiparib or olaparib for 48 h (A, B) or 5 d (C–E). Treated cells were then subjected to PI-staining flow cytometry analyses for cell cycle arrest (A, B) and PI/Annexin V-FITC double-staining flow cytometry analyses for apoptosis (C, D). Left, representative images with values representing the percentage of apoptotic cells; right, mean±SD from three independent experiments. Caspase 3/7 activity was determined by Caspase-Glo® 3/7 assays and the data expressed as the mean±SD from three independent experiments (E).