Table 1.
Cell Surface Proteins Elucidated as Potential Biomarkers Using Cell-SELEX and Aptamer-Based Targeted Proteomics
| target | aptamer | cell line | method | application |
|---|---|---|---|---|
| pigpen | III. I59 | YPEN-1, epithelial | aptamer-based affinity purification + SDS-PAGE + MS/MS59 | glioblastoma angiogenesis detection59 |
| TN-C | GBI-1077 | U251, glioblastoma | aptamer-based affinity purification + SDS-PAGE + LC-MS/MS77 | tumor diagnosis by MRI,78 cancer imaging79 |
| IGHM | TD0580 | Ramos, Burkitt’s lymphoma | 5-dUI photocross-linking + aptamer-based affinity purification + SDS-PAGE + LC-MS/MS74 | aptamer—micelles for cancer detection/drug delvery,81 cancer cell detection82, aptamer particles for circulating tumor cell detection,83 F-apt/SWNT for cancer cell probing,84 aptamer-mediated cell targeting68 |
| PTK7 | Sgc818 | CCRF-CEM, acute lymphoblastic leukemia | aptamer-based affinity purification + SDS-PAGE + LC-MS/MS61 | activatable aptamer probe for in vivo cancer imaging,71 logic-gate based cancer theranostics,64,69,70 aptamer—drug conjugates for targeted therapy67 |
| CXorfl7, Gal-3, GPNMB, LPL, SGP-1, LACTB/CD80, CD40, CPNE2 | not identified17 | immature dendritic/mature dendritic | aptamer-based affinity purification + SDS-PAGE + LC-MS/MS17 | immunological studies and clinical applications of DC-based cancer vaccines 17 |
| hemagglutinin | PP385 | vaccinia virus infected HeLa | AlphaScreen85 | diagnostic and/or therapeutic tools for infectious disease85 |
| Axl | GL21.T86 | U87MG, glioma | phospho-receptor tyrosine kinase array analysis + filter binding analysis87 | Axl inhibitor,87 aptamer-miRNA conjugate for targeted delivery88 and therapy89 |
| ALPPL-2 | SQ-290 | Panc-1 and Capan-1, pancreatic cancer | aptamer-based affinity purification + SDS-PAGE + LC-MS/MS90 | 5-fluoro-2′-deoxyuridine targeted delivery91 |
| troponin T | AraHH00192 | mTECs, mouse tumor endothelial | aptamer-based affinity purification + SDS-PAGE + MALDI-TOF93 | antiangiogenic therapy techniques93 |
| STIP1 | TOV694 | TOV-21G, ovarian cancer | formaldehyde cross-linking + aptamer-based affinity purification + SDS-PAGE + LC-MS75 | therapeutic tool75 |
| Siglec-5 | K1995 | NB4, acute myelogenous leukemia | aptamer-based affinity purification + SDS-PAGE + LC-MS/MS95 | detection of small numbers of AML cells in human bone marrow specimens95 |
| CD 109 | S396 | 5–8F, nasopharyngeal carcinoma | aptamer-based affinity purification + SDS-PAGE + LC-MS/MS96 | cellular imaging96 |
| Selectin L/integrin α 4 | sgc-3b/sgc-4e18 | Jurkat 6 × 10−1, acute lymphoblastic leukemia | formaldehyde cross-linking + SILAC + aptamer-based affinity purification + SDS-PAGE + LC-MS76 | cellular detection76 |