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. Author manuscript; available in PMC: 2017 Jul 20.
Published in final edited form as: Cell Rep. 2017 May 30;19(9):1929–1939. doi: 10.1016/j.celrep.2017.05.025

Figure 1. SOAR mutants 4KA or F394H interact with Orai1 channels in a state dependent manner.

Figure 1

(A) Cartoon representation of Orai1 and S1C mutants used in this work. (B) Summary of current densities recorded from cells co-expressing the indicated mCherry-S1C mutants together with Orai1-EGFP. (C) Summary of current densities recorded from cells co-expressing the indicated mCherry-S1C construct together with Orai1-S-EGFP. Note that mutations are introduced to S1C domains but not to the S domain. (D–F) Western blot analysis of cell lysate or immunoprecipitated material (IP) prepared from cells expressing Orai1-EGFP (D), Orai1-S-EGFP (E) or the indicated Orai1-SEGFP constructs (F) together with the indicated FLAG-mCherry-S1C constructs. Agarose beads conjugated to anti-mCherry nanododies were used to immunoprecipitate mCherry tagged proteins and antibodies against Orai1, FLAG and GFP were used for protein detection. Full blots of images shown in (D–F) and statistical analysis of data shown in (B–C) are displayed in Figure S1iii and in Figure S1ii, respectively.