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. Author manuscript; available in PMC: 2017 Jul 20.
Published in final edited form as: Cell Rep. 2017 May 30;19(9):1929–1939. doi: 10.1016/j.celrep.2017.05.025

Figure 3. Orai1 P245L interacts with S1C mutants.

Figure 3

(A) Western blot analysis of cell lysate or IP material prepared from cells expressing the indicated EGFP-S1C constructs together with mCherry-Orai1 P245L. Agarose beads conjugated to anti-GFP antibodies were used to immunoprecipitate EGFP tagged S1C proteins and antibodies against FLAG and mCherry were used for protein detection. (B) Time course of averaged currents (left) and typical plots of the current-voltage relationship (right) of currents recorded from cells co-expressing mCherry-orai1 P245L alone or with EGFP-S1C construct. Full blots of images shown in (A) and statistical analysis of data shown in (B) are displayed in Figure S3