. Author manuscript; available in PMC: 2018 Jun 22.

Published in final edited form as: Cell Chem Biol. 2017 Jun 8;24(6):758–766.e3. doi: 10.1016/j.chembiol.2017.05.018

Table 1.

Spectral properties of NIR FPs and their effective brightness in various mammalian cells. See also Figures 2, S1 and Table S1.

NIR FP	Ex, nm	Em, nm	Extinction coefficient, M⁻¹cm⁻¹	Quantum yield, %	NIR FP brightness in live cells relative to miRFP670: co-expression with EGFP downstream of T2A from the same plasmid (co-expression with EGFP from two separate plasmids), %
NIR FP	Ex, nm	Em, nm	Extinction coefficient, M⁻¹cm⁻¹	Quantum yield, %	HeLa	HEK293	U87	COS-7	U-2 OS
smURFP	642	670	180,000	18	1.9 (0.7)	0.8 (0.8)	1.2 (2.4)	1.7 (1.6)	0.6 (0.9)
miRFP670	642	670	87,400	14	100 (100)	100 (100)	100 (100)	100 (100)	100 (100)
mIFP	683	704	82,000	8.4	11 (26)	12 (29)	16 (28)	14 (44)	15 (38)
miRFP703	674	703	90,900	8.6	50 (68)	50 (69)	57 (58)	67 (71)	62 (89)