(A) A representative GUV in the presence of NLP1 exhibiting targeted binding to the Lo domain. GUVs in this sample were synthesized with a lipid composition consisting of 50% DOPC, 30% DPPC, 2% DPIDA, and 18% cholesterol. (B) A representative GUV before and after NLP1 binding exhibiting crowding induced mixing. GUVs in this sample were synthesized with a lipid composition consisting of 50% DOPC, 22% DPPC, 10% DPIDA, and 18% cholesterol. Scale bars are 20 µm.