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. Author manuscript; available in PMC: 2017 Jul 20.
Published in final edited form as: Methods Mol Biol. 2016;1364:261–275. doi: 10.1007/978-1-4939-3112-5_21

Figure 1.

Figure 1

Intra-jugular injection of AAV9-RNAi. A) An incision is made in the upper chest and neck of the mouse. Arrow indicates the incision. B) The jugular vein and pectoral muscle are exposed. C) The pectoral muscle is stabilized using forceps and a 30G Hamilton syringe is inserted through the pectoral muscle and into the jugular vein lumen. AAV9 vector is infused into the vein. D) Close-up image of jugular vein injection with needle tip inside of the vein lumen (arrow = jugular vein; arrowhead = pectoral muscle). E) The skin is pinched and pulled away from the body and closed with wound clip. F) The incision is completely closed with wound clips.