Figure 4.
Proximal but not distal Gαq signaling cross-talk is involved in transmitting Ang II-induced conformational information to FP. A and B, HEK 293 cells were transfected with FP-ICL3 P4-RLucII and AT1R-WT. Cells were pre-treated with 1 μm of the PKC inhibitor Gö6983 for 1 h (A) or co-stimulated with 100 nm phorbol 12-myristate 13-acetate (B), a direct PKC activator. Cells were stimulated with 1 μm PGF2α or Ang II and the change in BRET (ΔBRET) due to ligand stimulation is reported. D, HEK 293 cells were transfected with FP-ICL3 P4-RLucII and AT1R-WT. Cells were pre-treated with 1 μm U73122 for 1 h. Cells were stimulated with 1 μm PGF2α or Ang II and the change in BRET (ΔBRET) due to ligand stimulation is reported. Bars represent the mean of 3 independent biological replicates and error bars represent S.E. A t test was performed to compare buffer versus treatment where ** = p < 0.01.