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Coimmunoprecipitation of the exosome with Rrp6-TAP. Total extract was incubated with IgG-Sepharose beads, and proteins were eluted with TEV protease. Samples from total extract (input (In)), flow-through (FT), and elution (Elu) were subjected to SDS-PAGE and silver-stained (A). The exosome subunits were identified by mass spectrometry. B, Western blot of the same samples with anti-CBP. Rrp6-TAP bound efficiently to the resin and was eluted after cleavage with TEV protease.
