Figure 2.

Proteins co-purified with Rrp6-TAP were subjected to gel filtration on a Superose 6 column for the separation of the exosome complex. A, chromatographic profile of the proteins. Arrows indicate the elution volumes of the molecular mass controls run through the same column. B, SDS-PAGE of the fractions obtained. Fractions were also analyzed by mass spectrometry for the identification of the proteins. C, fractions 29–32 contained, in addition to exosome subunits, some exosome cofactors, karyopherins, and chaperones, grouped in different colors. D, proteins co-purifying with Rrp6-TAP were classified by GO using DAVID software. Karyopherins were the second most predominant category of proteins. mAU, milli-absorbance units.