Figure 5.

Deletion of KAP104 does not affect localization of GFP-Rrp6 at 37 °C. A, fluorescence microscopy for the analysis of the subcellular localization of GFP-Rrp6 in Δkap104 cells. GFP-Nab2, a protein affected by the absence of Kap104, was used as a control. Analysis of GFP-Rrp6 and GFP-Nab2 relative to PI in Δkap104 cells growing at 37 °C by is shown on the right. B, Western blotting of total cell extract from Δkap104 cells expressing GFP-Rrp6 or GFP-Nab2 incubated at 25 or 37 °C was performed with antibody against GFP. Expression levels of GFP-Rrp6, but not those of GFP-Nab2, decreased at 37 °C in Δkap120 cells. Antibody against Arp2 was used as an internal control.