Figure 6.

Expression of Rrp6 deletion mutants in Δrrp6. A, schematic representation of the deletion mutants of Rrp6. Rrp47 and core exosome interaction regions as well as active domains of Rrp6 are highlighted. Positions of putative NLSs are shown in blue. B, Western blot for the determination of the expression of the GFP-Rrp6 mutants in the Δrrp6 strain. Arp2 was used as an internal control. C, analysis of growth of Δrrp6 cells expressing either GFP or the different GFP-Rrp6 constructs at 30 or 37 °C. Mutants lacking the canonical NLS, Rrp6(1–619) and Rrp6(1–693), complement growth of Δrrp6 at 37 °C. HRDC, helicase and RNase D C-terminal domain.