Figure 9.

Analysis of the subcellular localization of Rrp6 or Rrp6 mutants in Δrrp47 cells. A, fluorescence microscopy for the analysis of the subcellular localization of GFP-Rrp6 or the mutants containing the Rrp47-interacting domain, Rrp6(1–106), Rrp6(1–398), Rrp6(1–619), andRrp6(1–693), in Δrrp47 cells. Absence of Rrp47 affects localization of the Rrp6(1–106) and Rrp6(1–398) mutants. B, analysis of the expression levels of Rrp6 deletion mutants in Δrrp47 cells by Western blotting. Mutants are expressed at higher levels than full-length Rrp6. Arp2 was used as an internal control. C, analysis of growth of Δrrp47 cells expressing either GFP or the different GFP-Rrp6 constructs at 25 or 37 °C. Full-length Rrp6 and mutants Rrp6(1–619) and Rrp6(1–693) complement growth of Δrrp47 at 37 °C.