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. 2017 May 26;292(29):12324–12338. doi: 10.1074/jbc.M116.772624

Figure 7.

Figure 7.

Loss of a single SODA allele impairs metacyclogenesis. A, infective metacyclic forms were quantified microscopically from stationary (day 7) WT, SODAsodA, or SODAsodA + SODA cultures after agglutinating promastigotes with the m3A.1 monoclonal antibody. The data represent the mean ± S.D. of the percentage of metacyclics in triplicate determinations and represent data obtained from four independent experiments (**, p = 0.003; *, p = 0.01). B, SEM images of representative parasite morphologies observed in day 7 cultures of wild-type (WT), SODA single knock-out (SODAsodA), or SODA single knock-out complemented (SODAsodA + SODA) promastigotes. Arrowheads point to metacyclic forms. Bars, 4 μm. C, mitochondrial membrane potential (ψm) WT, SODAsodA, or SODAsodA + SODA parasites during different phases of growth determined by quantification of JC-1 uptake. The data represent the mean ± S.D. of three independent experiments. (Student's t test, **, p ≤ 0.01).