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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Hepatology. 2017 Jun 30;66(2):466–480. doi: 10.1002/hep.29230

Figure 5.

Figure 5

AEG-1 inhibits expression of PPARα target genes and fatty acid β-oxidation. A–C. Analysis of PPARα target genes in WT and Alb/AEG-1 hepatocytes (A), AEG-1+/+ and AEG-1−/− hepatocytes (B) with or without PPARα ligands, and in chow- and HFD-fed WT and Alb/AEG-1 livers (C). Normalized by GAPDH. D. Measurement of 3′-HB level in mice sera and hepatocytes. For A–D, n = 5/group. Data represent mean ± SEM of at least 3 experiments. *: p<0.01. E. Fatty acid β-oxidation rate measured by acid soluble metabolite (ASM) and CO2 in the indicated livers. AICAR is an inducer. Data represent mean ± SEM of at least 3 experiments. *: p<0.01 between WT and Alb/AEG-1. F. ChIP assay to check the binding of the indicated proteins on PPARα targets Acox1 and Cpt1a promoter in WT and Alb/AEG-1 hepatocytes using IgG as control. Data represent mean ± SEM of at least 3 experiments. *: p<0.01.