Table 1. Stereology Study Parameters.
Parameters reported for the stereology analysis of Complexin3 (at P8) and Foxp1 (at P8 and P21). Same counting parameters were used for control and injured hemispheres; estimates are reported for control and in parentheses HI. Estimated population (number weighted section thickness) is reported for mean N.
| Parameters | P8, Cplx3 | P8, Foxp1 | P21, Foxp1 |
|---|---|---|---|
| observed group mean, Mean N | 35457 (26747) | 1234640 (1195085) | 1195632 (1265190) |
| observed coefficient of variation of group mean, OCV | 0.23 (0.30) | 0.14 (0.37) | 0.023 (0.038) |
| number of individuals in group, n | 5 (8) | 5 (8) | 8 (7) |
| observed coefficient of variation of estimator, CE | 0.08 (0.10) | 0.08 (0.08) | 0.05 (0.05) |
| section sampling fraction, ssf | 1/12 | 1/12 | 1/12 |
| fraction of area of section sampled, asf | 0.1024 | 0.0028 | 0.0064 |
| thickness sampling fraction (h/t), tsf | 0.61 | 0.66 | 0.57 |
| disector volume, h x Aframe | 16000 | 4000 | 16000 |
| average number of objects counted in each individual, ΣQ− | 208 | 204 | 366 |
| thickness of mounted section, t | 16.3μm | 15μm | 17.4 μm |
| height of disector, h | 10 μm | 10 μm | 10 μm |
| guard zone | 1μm | 1μm | 1μm |
| smoothness factor, m | 1 | 1 | 1 |
| number of sections used, i | 5 | 5 | 8 |
| counting frame, Aframe | 40×40 μm2 | 20×20 μm2 | 40×40 μm2 |
| sampling grid area, Agrid | 125×125 μm2 | 375×375 μm2 | 500×500 μm2 |
*
for the first 4 rows, numbers are reported as: control hemisphere (HI injured hemisphere)