Table 2.
Immunohistochemical analysis by treatment group
| Target | Ab | Treatment | Hippocampus | Corona Radiata | Cortex | Cerebellum (White & Grey matter) | |
|---|---|---|---|---|---|---|---|
| Neurons | NeuN | Control | 78.34±6.01 | 27.73±5.19 | 146.1±18.14* | 94.93±4.94** | |
| UCO | 73.06±4.52 | 23.17±8.54 | 107.3±5.92 | 63.48±6.22 | |||
| UCO+TH | 72.52±7.43 | 31.40±6.07 | 109.1±13.93 | 82.47±8.04 | |||
| UCO+TH+Epo | 79.06±4.72 | 24.57±4.26 | 110.5±9.19 | 73.24±7.29 | |||
| Oligoden-drocytes | Olig2 | Control | 148.5±32.50 | 300.4±38.02 | 355.4±27.77 | 356.40±42.88* | |
| UCO | 118.0±36.29 | 246.2±24.07 | 352.8±41.52 | 243.5±27.80 | |||
| UCO+TH | 213.0±35.68 | 242.8±34.26 | 320.6±37.11 | 304.0±27.10 | |||
| UCO+TH+Epo | 188.5±32.50 | 272.6±19.95 | 314.8±41.30 | 282.4±37.64 | |||
| Myelin | MBP | Control | 17.25±2.49* | 61.80±36.08* | 17.07±2.24 | 69.67±6.03 | |
| UCO | 10.40±0.99 | 34.92±3.38 | 15.92±2.42 | 58.85±6.06 | |||
| UCO+TH | 12.42±1.22 | 30.00±0.87 | 18.33±3.15 | 68.93±6.08 | |||
| UCO+TH+Epo | 27.53±3.23### | 23.81±2.71# | 16.06±0.97 | 64.95±7.89 | |||
| Astrocytes | GFAP | Control | 39.94±6.43 | 55.27±1.64 | 31.47±4.55 | 66.20±5.86*** | |
| UCO | 33.22±3.83 | 50.63±2.42 | 29.17±3.57 | 34.70±2.01 | |||
| UCO+TH | 39.67±2.51 | 58.10±2.45 | 28.90±4.57 | 68.86±3.14§§§ | |||
| UCO+TH+Epo | 34.83±2.12 | 57.62±4.24 | 31.33±2.52 | 54.95±3.89### | |||
| Microglia | Iba1 | Control | 25.78±4.74 | 18.00±2.09 | 20.80±3.61 | 11.47±1.58 | |
| UCO | 16.61±2.75 | 19.52±0.60 | 19.33±1.96 | 11.91±1.38 | |||
| UCO+TH | 26.83±3.55 | 19.71±0.68 | 19.67±0.43 | 11.91–1.38 | |||
| UCO+TH+Epo | 19.45±4.10 | 18.28±3.18 | 20.97±1.29 | 8.05±0.77 | |||
| Macrophage/Microglia | CD68 | Control | 19.09±2.79* | 15.60±2.34 | 12.27±2.86 | 20.41±0.99 | 5.10±1.03 |
| UCO | 10.19±2.96 | 15.09±2.69 | 11.72±1.25 | 21.65±3.08 | 8.16±2.13 | ||
| UCO+TH | 14.47±4.04 | 22.13±3.74 | 10.73±1.19 | 33.20±7.68 | 6.20±1.21 | ||
| UCO+TH+Epo | 16.36±1.38 | 12.12±1.79 | 13.95±2.22 | 20.41±2.03 | 4.64±0.50 | ||
| Proliferation | Ki67 | Control | 3.00±0.86 | 6.87±2.11 | 0.33±0.11* | 0.00±0.00 | 0.50±0.50 |
| UCO | 9.91±5.83 | 12.26±2.96 | 2.15±0.39 | 1.79±0.74 | 2.67±1.84 | ||
| UCO+TH | 3.87±1.08 | 6.40±2.07 | 1.86±0.34 | 1.40±0.60 | 0.40±0.40 | ||
| UCO+TH+Epo | 2.16±0.65 | 4.99±1.76 | 1.55±0.32 | 1.14±0.60 | 1.00±0.65 | ||
Ab=antibody. UCO=umbilical cord occlusion. TH=therapeutic hypothermia. Epo=erythropoietin. Cerebella analysis made across the white and gray unless specified.
*,**, **
; significance in Mann-Whitney U test of UCO vs Control. Where p<0.05; ANOVA comparing UCO, UCO+TH & UCO+TH+ Epo then §,§§,§§§ designates the level of significance in a Dunnets post-test of UCO vs UCO+TH and #,##,### designates the level of significance in post-test of UCO vs UCO+TH+Epo.