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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Hepatology. 2017 Jul 4;66(2):602–615. doi: 10.1002/hep.29144

Figure 1. Small specific-sized HA differentially influence LPS-stimulated cytokine expression in primary cultures of rat Kupffer cells from ethanol- and pair-fed rats.

Figure 1

Wistar rats were allowed free access to a Lieber-DeCarli ethanol diet or pair-fed control diet for 4 weeks. Kupffer cells were isolated and cultured overnight. Kupffer cells were then treated with 100μg/ml specific-sized HA fragments for 5h and then challenged with 10ng/ml LPS for an additional 1hr. Expression of TNFα (A), IL6 (B) and CXCL10 (C) mRNA was measured by qRT-PCR and normalized to 18S rRNA. (A) n=10–14 and (B/C) n=4–5.