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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Jan 20;1863(10 Pt B):2584–2593. doi: 10.1016/j.bbadis.2017.01.014

Figure 3.

Figure 3

Apoptosis of CD4+ and CD8+ T cells in the spleens of septic aged rats. Aged rats were subjected to sham operation or CLP with vehicle (normal saline) or GG (80 nmol/kg of Ghr and 50 μg/kg GH) treatment at 5 h after CLP. Spleens were harvested at 20 h after CLP. Sections of splenic tissues were immunostained for CD4 and CD8 (A). In another group of animals, isolated splenocytes were stained with PE/Cy7-labeled anti-CD4 or APC-labeled anti-CD8 antibodies followed by FITC-labeled TUNEL reaction and analyzed by flow cytometry (B–C). Isotype-matched antibodies were used as negative controls. Data are expressed as mean ± SEM (n = 5–8 per group). *P < 0.05 versus sham and #P < 0.05 versus vehicle-treated septic animals.