Fig. 4.

Map-based cloning of the sug-h mutant. a Candidate region of the sugary gene was on the long arm of chromosome 8. Candidate gene of the first bulked segregant analysis, Os08g40930, was located on the AP005509 BAC clone and contained 18 exons (black boxes) and 17 introns (white boxes). A point mutation, G to A, occurred in the 15th exon (red box). b Schematic representation of OsBEIIa on the long arm of chromosome 4. Candidate region of the gene related to the sug-h mutant was located within the AL731641 clone. The physical distance was approximately 12.9 kb, which included three candidate genes. Schematic structure of the candidate gene, Os04g33460, contained 22 exons (black boxes) and 21 introns (white boxes). A point mutation, G to A, occurred in the 13th exon (red box). Gray arrows indicate the main genes with known functions within the candidate region. Red arrow indicates the candidate gene. ATG and TGA/TAA indicate the initiation and termination codons, respectively