Skip to main content
. 2017 Jul 21;8:173. doi: 10.3389/fendo.2017.00173

Figure 2.

Figure 2

Activating transcription factor-5 (ATF5) knockdown decreases the proliferation rate and Akt activation in mammary tumor cells. Scrambled and knockdown of ATF5 (ATF5-KD) cells, derived from (A) Mvt1 cell line or (B) Met1 cell line, were grown for 72 h in 96-well plates and subjected to proliferation assay analysis. Proliferation fold was calculated as the ratio between the fluorescent values at the end of the experiment and time 0. Results are representatives of two independent experiments and presented as mean ± SEM, n = 4; Student’s t-test was used to determine statistical significance; ***P < 0.001. (C) Protein was extracted from scrambled and ATF5-KD Mvt1 cells, and Western blot analysis was performed using antibodies directed against ATF5, phospho-Akt (Thr308), total-Akt, and β-actin. (D) Densitometry analysis was performed using the ImageQuant software. Results represent mean ± SEM in arbitrary units (A.U.) of four independent experiments, n = 4; Student’s t-test was used to determine statistical significance; ***P < 0.001.