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Establishment of NK cells expressing a reporter gene. (A) RT-polymerase chain reaction and (B) western blotting analyses showing effluc expression at the mRNA and protein levels, respectively, in NK/F cells. (C,D) Firefly luciferase activity was determined by bioluminescence imaging, and the intensity of the effluc was correlated with the cell number, R2 = 0.91. effluc, enhanced firefly luciferase; Rluc, Renilla luciferase; NK/F, NK-92MI/effluc cells.
