Figure 5.

NK cell-based immunotherapy for anaplastic thyroid cancer (ATC) pulmonary metastasis. CAL-62/F cells were injected intravenously via the tail vein of nude mice, and after 7 days, NK cells were intravenously injected. After the in vivo experiment, the mice were sacrificed, and ex vivo experiments were performed. (A) In vivo bioluminescence imaging (BLI) of an ATC tumor. (B) Quantification of the BLI signals. (C) Hematoxylin and eosin staining of the ATC pulmonary metastases. (D) Ex vivo BLI of the ATC pulmonary metastases. Experiments were performed at least in triplicate, and the values represent the mean ± SE. *p < 0.05, **p < 0.01. effluc, enhanced firefly luciferase; CAL-62/F, CAL-62/effluc cells; NK, NK-92MI cells.