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. 2017 Jul 20;7:5948. doi: 10.1038/s41598-017-06183-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic tree reconstruction of Tripneustes control region (CR) sequences. The BI tree is based on 19 unique haplotypes, 446 bp long, representing T. kermadecensis n. sp., T. g. elatensis and T. g. gratilla, and is rooted on Hemicentrotus pulcherrimus and Strongylocentrotus droebachiensis (GenBank accession numbers KC490911, EU054306 and NC009940, respectively). Color code as in Fig. 5. Supporting values (>0.9 posterior probabilities and >60% ML bootstrap values) are shown above the nodes. Clades A (comprising T. kermadecensis n. sp.), and B (comprising both T. g. gratilla and T. g. elatensis) are discussed in the text. Details on the sequences used for this tree are given in the Supplementary Tables S1 and S2.