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. 2017 Jul 20;7:5986. doi: 10.1038/s41598-017-06236-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic characterization and intranodal distribution of Ly6C^hi monocytes. (A) Phenotypic characterization of Ly6C^hi monocytes in the draining lymph node in response to subcutaneous CpG injection. The levels of CD11c, MHCII, CD86, CD40 and CD64 of ly6C^hi monocytes are shown at 0 h, 24 h and 48 h after subcutaneous CpG injection. (B) Confocal images of popliteal lymph nodes in steady state and following footpad injection of CpG. Sections were stained for CD3 (blue), CD11c (red) and CD64 (green) to visualize T cells, DCs and Ly6C^hi monocytes.